Journal: Frontiers in physiology
Article Title: NF-κB-dependent repression of Sox18 transcription factor requires the epigenetic regulators histone deacetylases 1 and 2 in acute lung injury.
doi: 10.3389/fphys.2022.947537
Figure Lengend Snippet: FIGURE 1 Effects of class I HDACs selective inhibitors on LPS-induced endothelial barrier disruption in cultured human lung microvascular endothelial cells. HLMVEC monolayers grown in ECIS arrays were pre-treated with HDAC inhibitors or vehicle for 30 min then challenged with LPS. TER was recorded in real time to evaluate possible barrier-protective effects of HDAC inhibitors. Data are shown for tacedinaline (HDAC -1 and -3 inhibitor) (A), FK228 (HDAC -1 and -2 inhibitor) (B), MI192 (HDAC -2 and -3 inhibitor) (C), and RGFP966 (HDAC3 inhibitor) (D). Data are mean ± SEM, n = 4. *p < 0.05 vs. Control; †p < 0.05 vs. LPS alone.
Article Snippet: The cells were grown in 8-well 8W10E ECIS arrays (Applied BioPhysics) to 100%-confluency in complete EGM-2 medium.
Techniques: Disruption, Cell Culture, Control